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Validation of Two Commercial Lateral Flow Devices for the Detection of Peanut Proteins in Cookies: Interlaboratory Study

2006; Oxford University Press; Volume: 89; Issue: 2 Linguagem: Inglês

10.1093/jaoac/89.2.462

1944-7922

Arjon J. van Hengel, Claudia Capelletti, Marcel Brohée, Elke Anklam, M-C S Baumgartner, Michael Abbott, Sabine Baumgartner, M.G.E.G. Bremer, Dean Clarke, Chantal Cléroux, Claude Demeulemester, F Eckhart, É. Gelencsér, Susan L. Hefle, H Herrnegger, H Hörtner, S Kyriakidis, Rob Lowe, G. Marx, W Mayer, Albrecht Paschke, M-C. Robert, K. Schneede, Robert R. Sherlock, D. Suter, K. Warner, Mads U. Werner,

Identification and Quantification in Food

Results are reported for an interlaboratory validation study of 2 commercially available Iateral flow devices (dipstick tests) designed to detect peanut residues in food matrixes. The test samples used in this study were cookies containing peanuts at 7 different concentrations in the range of 0-30 mg peanuts/kg food matrix. The test samples with sufficient and proven homogeneity were prepared in our laboratory. The analyses of the samples (5 times per level by each laboratory) were performed by 18 laboratories worldwide which submitted a total of 1260 analytical results. One laboratory was found to be an outlier for one of the test kits. In general, both test kits performed well. However, some false-negative results were reported for all matrixes containing < 21 mg peanuts/kg cookie. It must be stressed that the test kits were challenged beyond their cut-off limits (> or = 5 mg/kg, depending on the food matrix). One test kit showed fewer false-negative results, but it led to some false-positive results for the blank materials. The sensitivity of the dipstick tests approaches that achieved with enzyme-linked immunosorbent assays.