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Tetanus toxin light chain expression in Sertoli cells of transgenic mice causes alterations of the actin cytoskeleton and disrupts spermatogenesis.

1993; Springer Nature; Volume: 12; Issue: 9 Linguagem: Inglês

10.1002/j.1460-2075.1993.tb06010.x

1460-2075

Ulrich Eisel, Kay Reynolds, Michelle Riddick, Andreas Zimmer, Heiner Niemann, Andreas Zimmer,

Biochemical and Structural Characterization

Research Article1 September 1993free access Tetanus toxin light chain expression in Sertoli cells of transgenic mice causes alterations of the actin cytoskeleton and disrupts spermatogenesis. U. Eisel U. Eisel Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author K. Reynolds K. Reynolds Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author M. Riddick M. Riddick Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author A. Zimmer A. Zimmer Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author H. Niemann H. Niemann Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author A. Zimmer A. Zimmer Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author U. Eisel U. Eisel Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author K. Reynolds K. Reynolds Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author M. Riddick M. Riddick Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author A. Zimmer A. Zimmer Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author H. Niemann H. Niemann Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author A. Zimmer A. Zimmer Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. Search for more papers by this author Author Information U. Eisel1, K. Reynolds1, M. Riddick1, A. Zimmer1, H. Niemann1 and A. Zimmer1 1Developmental Biology Unit, National Institute of Mental Health, Bethesda, MD 20892. The EMBO Journal (1993)12:3365-3372https://doi.org/10.1002/j.1460-2075.1993.tb06010.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Tetanus toxin is a powerful neurotoxin known to inhibit neurotransmitter release. The tetanus toxin light chain is a metalloprotease that cleaves some members of the synaptobrevin gene family with high specificity. Here, we report the expression of a synthetic gene encoding the tetanus toxin light chain in the seminiferous epithelium of transgenic mice. Spermatogenesis was severely impaired and mature spermatozoa were completely absent. Late spermatids exhibited pleomorphic shapes and acrosomal distortions. The number of Leydig cells was greatly increased. In situ hybridization analysis revealed that the toxin acts on Sertoli cells. Affected cells exhibited an aberrant distribution of actin filaments and many cells contained large vacuoles. Our results demonstrate that tetanus toxin is active in non-neuronal cells and suggest an important function for members of the synaptobrevin gene family during the late stages of spermatogenesis. Previous ArticleNext Article Volume 12Issue 91 September 1993In this issue RelatedDetailsLoading ...