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Sterol metabolism studies in the rat

1977; Elsevier BV; Volume: 487; Issue: 2 Linguagem: Inglês

10.1016/0005-2760(77)90005-4

1879-145X

Bertram I. Cohen, Robert F. Raicht, Erwin H. Mosbach,

Receptor Mechanisms and Signaling

Sterol metabolism studies using a combination of thin-layer chromatography, gas-liquid chromatography and isotopic methods were carried out in rats fed diets containing: (a) control chow (group 1); (b) 0.8% β-sitosterol (group 2); (c) 0.8% β-sitosterol plus 0.5% sodium taurochenodeoxycholate (group 3); and (d) 0.8% β-sitosterol plus 0.5% sodium taurocholate (group 4). Feeding β-sitosterol led to an inhibition of cholesterol absorption, increased cholesterol output (cholesterol balance, 28.8 mg/day vs. 19.5 mg/day in controls) and no significant increase in bile acid synthesis. Rats receiving sodium taurochenodeoxycholate or sodium taurocholate in addition to β-sitosterol showed: (a) decreased levels of bile acid synthesis; (b) low cholesterol absorption; and (c) increased output of fecal and endogenous neutral sterols. Levels of cholesterol in plasma were not significantly different from those in the control animals. Liver cholesterol levels were significantly lower in the taurochenodeoxycholate plus β-sitosterol (group 3) vs. the taurocholate plus β-sitosterol animals (group 4). However, biliary cholesterol concentrations were elevated in both bile acid-plant sterol groups (0.38 mg/ml in group 3; 0.44 mg/ml in group 4 vs. 0.19 mg/ml in group 1). There were no significant differences in sterol metabolism between groups of rats fed sodium taurochenodeoxycholate plus β-sitosterol compared to rats fed sodium taurocholate plus β-sitosterol.