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A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase.

1992; Springer Nature; Volume: 11; Issue: 2 Linguagem: Inglês

10.1002/j.1460-2075.1992.tb05082.x

1460-2075

Katsuhiko Tsukamoto, Ian J. Jackson, Kazunori Urabe, Paul Montague, Vincent J. Hearing,

Skin Protection and Aging

Research Article1 February 1992free access A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. K. Tsukamoto K. Tsukamoto Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author I.J. Jackson I.J. Jackson Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author K. Urabe K. Urabe Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author P.M. Montague P.M. Montague Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author V.J. Hearing V.J. Hearing Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author K. Tsukamoto K. Tsukamoto Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author I.J. Jackson I.J. Jackson Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author K. Urabe K. Urabe Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author P.M. Montague P.M. Montague Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author V.J. Hearing V.J. Hearing Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Search for more papers by this author Author Information K. Tsukamoto1, I.J. Jackson1, K. Urabe1, P.M. Montague1 and V.J. Hearing1 1Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. The EMBO Journal (1992)11:519-526https://doi.org/10.1002/j.1460-2075.1992.tb05082.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The production of melanin pigment in mammals requires tyrosinase, an enzyme which hydroxylates the amino acid tyrosine to DOPA (3,4-dihydroxyphenylalanine), thus allowing the cascade of reactions necessary to synthesize that biopolymer. However, there are other regulatory steps that follow the action of tyrosinase and modulate the quantity and quality of the melanin produced. DOPAchrome tautomerase is one such melanogenic enzyme that isomerizes the pigmented intermediate DOPAchrome to DHICA (5,6-dihydroxyindole-2-carboxylic acid) rather than to DHI (5,6-dihydroxyindole), which would be generated spontaneously. This enzyme thus regulates a switch that controls the proportion of carboxylated subunits in the melanin biopolymer. Efforts to clone the gene for tyrosinase have resulted in the isolation of a family of tyrosinase related genes which have significant homology and encode proteins with similar predicted structural characteristics. Using specific antibodies generated against synthetic peptides encoded by unique areas of several of those proteins, we have immuno-affinity purified them and studied their melanogenic catalytic functions. We now report that TRP-2 (tyrosinase related protein-2), which maps to and is mutated at the slaty locus in mice, encodes a protein with DOPAchrome tautomerase activity. Previous ArticleNext Article Volume 11Issue 21 February 1992In this issue RelatedDetailsLoading ...