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Determination of the Appetite Suppressant P57 in Hoodia gordonii Plant Extracts and Dietary Supplements by Liquid Chromatography/Electrospray Ionization Mass Spectrometry (LC-MSD-TOF) and LC-UV Methods

2006; Oxford University Press; Volume: 89; Issue: 3 Linguagem: Inglês

10.1093/jaoac/89.3.606

1944-7922

Bharathi Avula, Yan‐Hong Wang, Rahul S. Pawar, Yatin J. Shukla, Brian T Schaneberg, Ikhlas A. Khan,

Phytochemical Studies and Bioactivities

Hoodia gordonii is traditionally used in South Africa for its appetite suppressant properties. P57AS3 (P57), an oxypregnane steroidal glycoside, is the only reported active constituent from this plant as an appetite suppressant. Effective quality control of these extracts or products requires rapid methods to determine P57 content. New methods of liquid chromatography/mass spectrometry (LC/MS) and LC-UV for analysis of P57 from H. gordonii have been developed. The quantitative determination of P57 was achieved with a Phenomenex Gemini (Torrance, CA) reversed-phase column using gradient mobile phase of water and acetonitrile, both containing 0.1% acetic acid. The method was validated for linearity, repeatability, and limits of detection and quantification. Good results were obtained in terms of repeatability (relative standard deviation <5.0%) and recovery (98.5-103.5%). The developed methods were applied to the determination of P57 for H. gordonii plant samples, one related genus (Opuntia ficus-indica), and dietary supplements that claim to contain H. gordonii.