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Production of a Lactate-Associated Galactan Sulfate by a Dinoflagellate <i>Gymnodinium</i> A₃

1995; Springer Science+Business Media; Volume: 61; Issue: 2 Linguagem: Inglês

10.2331/fishsci.61.321

1444-2906

M Hasui, Masahiro Matsuda, Sadaaki Yoshimatsu, Koichi Okutani,

Marine Toxins and Detection Methods

A marine microalga, Gymnodinium A3 OKU-1 strain, which was originally isolated from a water sample of the Seto Inland Sea produced an extracellular acidic polysaccharide when grown in a seawater medium. Isolation and purification of this polysaccharide were accomplished by precipitation with ethanol and Cetavlon, followed by fractionation with DEAE-cellulose column chromatography. This polysaccharide preparation, which was homogeneous in both ultracentrifugal and electrophoretic analyses, was composed of galactan containing sulfate and lactate as the components.