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BIBTEX RIS

Primary structure of the carbohydrate chain of soybean agglutinin. A reinvestigation by high resolution 1H NMR spectroscopy.

1981; Elsevier BV; Volume: 256; Issue: 15 Linguagem: Inglês

10.1016/s0021-9258(18)43329-7

ISSN

1083-351X

Autores

L. Dorland, H. van Halbeek, J.F. Vleigenthart, Halina Lis, Nathan Sharon,

Tópico(s)

Glycosylation and Glycoproteins Research

Resumo

it was concluded that the plant glycoprotein soybean agglutinin contains two types of N-glycosidically linked carbohydrate units, both consisting of mannose and N-acetylglucosamine, in a molar ratio of 9:2.Both were described to have the same core structure, Mana(l+ 6)[Mana(l+ 3)]ManP(l -+ 4)GlcNAc#l(l-+ 4)GlcNAc, but different patterns of branching.Here we present data showing that the asparagine-linked carbohydrate moiety of soybean agglutinin is homogeneous and possesses the following structure: Mana(1 + %)Mana(l-2) Mana(1-3) \ Manp(l+ 4)GlcNAcp(1+ 4)GlcNAc/3(1+ N)Asn Mana(1-2)Mana(l+ 3) \ / / Mana(l+ 6) Mana(1 + %)Mana(l+ 6)

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