Artigo Acesso aberto Revisado por pares

Effects of Taurine and Glycine on Epileptiform Activity Induced by Removal of Mg 2+ in Combined Rat Entorhinal Cortex–Hippocampal Slices

2003; Wiley; Volume: 44; Issue: 9 Linguagem: Inglês

10.1046/j.1528-1157.2003.01603.x

ISSN

1528-1167

Autores

Anne Kirchner, Jörg Breustedt, Berit Rosche, Uwe Heinemann, Volker Schmieden,

Tópico(s)

Pharmacological Receptor Mechanisms and Effects

Resumo

Summary: Purpose: The imbalance between neuronal inhibition and excitation contributes to epileptogenesis. Inhibition in the central nervous system (CNS) is mediated by γ‐aminobutyric acid (GABA) and glycine. Recent studies indicate the expression of glycine receptor (GlyR) in hippocampus and neocortex. However, the function of GlyR in these regions is not clarified completely. The aim of this study was to investigate whether the GlyR agonists glycine and taurine promote an anticonvulsive effect. Methods: We induced epileptiform discharges by reducing extracellular Mg 2+ concentration in combined rat entorhinal cortex–hippocampal slices (400 μm). Epileptiform discharges were detected by using extracellular recording techniques. Results: Seizure‐like events were suppressed by taurine, exhibiting a half‐maximal inhibitory effect (IC 50 ) of 0.9 m M . Suppression of late recurrent discharges in the medial entorhinal cortex and recurrent short discharges in the hippocampus was obtained at an IC 50 value of 1.6 and 2.1 m M , respectively. Strychnine at concentrations <1 μ M abolished these effects. Likewise glycine, after an initial proconvulsant effect, suppressed epileptiform discharges. Conclusions: These findings show that GlyR agonists, in particular taurine, could serve as potential anticonvulsants and suggest an important role of GlyR in cortical function and dysfunction.

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