miR‐1275: A single microRNA that targets the three IGF2‐mRNA‐binding proteins hindering tumor growth in hepatocellular carcinoma
2015; Wiley; Volume: 589; Issue: 17 Linguagem: Inglês
10.1016/j.febslet.2015.06.038
ISSN1873-3468
AutoresInjie Omar Fawzy, Mohammed Tarif Hamza, Karim Adel Hosny, Gamal Esmat, Hend Mohamed El Tayebi, Ahmed Ihab Abdelaziz,
Tópico(s)RNA Research and Splicing
ResumoThis study aimed to identify a single miRNA or miR (microRNA) which regulates the three insulin‐like growth factor‐2‐mRNA‐binding proteins (IGF2BP1, 2 and 3). Bioinformatics predicted miR‐1275 to simultaneously target the three IGF2BPs, and screening revealed miR‐1275 to be underexpressed in hepatocellular carcinoma (HCC) tissues. Transfection of HuH‐7 cells with miR‐1275 suppressed IGF2BPs expression and all three IGF2BPs were confirmed as targets of miR‐1275. Ectopic expression of miR‐1275 and knockdown of IGF2BPs inhibited malignant cell behaviors, and also reduced IGF1R protein and mRNA. Finally IGF1R was validated as a direct target of miR‐1275. These findings indicate that the tumor‐suppressor miR‐1275 can control HCC tumor growth partially through simultaneously regulating the oncogenic IGF2BPs and IGF1R.
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