Artigo Acesso aberto Revisado por pares

Structural study of the carbohydrate moiety of hen ovomucoid. Occurrence of a series of pentaantennary complex-type asparagine-linked sugar chains.

1982; Elsevier BV; Volume: 257; Issue: 21 Linguagem: Inglês

10.1016/s0021-9258(18)33585-3

ISSN

1083-351X

Autores

Koji Yamashita, Johannis P. Kamerling, Akira Kobata,

Tópico(s)

Proteoglycans and glycosaminoglycans research

Resumo

Hen ovomucoid contains a series of novel pentaantennary complex-type asparagine-linked sugar chains which can be classified into three groups, N-1, N-2, and N-3b.N-3b, the structure of which is found as a common 2 GlcNAcBl+~M.lanol' 1 GlcNAcBl, G1cNAcB1 4 G l C N A C B l h G1cNAcBIR f 3 'Ma~B1+4GlcNAcB1*4GlcNAc, 5 GlcNAcBl ,2Manal structure in all pentaantennary sugar chains.N-2 is a mixture of three isomeric monogalactosyl derivatives of N-3b, and N-1 is a mixture of two isomeric digalactosyl derivatives of N-3b.Most of the galactoses are plinked at the C-4 position of /3-N-acetylglucosamine residues 2 and 4 in the N-3b structure shown above.Small amounts of the galactoses are also linked at the C-4 position of B-N-acetylglucosamine residue 3. ' The abbreviation used is: X,yINAc, IV-acetylxylosami~~e. Subscript OT is used in this paper to indicate NaB['H]$-reduced sugars.All sugars mentioned in this paper were of D-configuration.

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