Pyruvate oxidase and oxaloacetate decarboxylase enzyme electrodes
1989; Elsevier BV; Volume: 222; Issue: 1 Linguagem: Inglês
10.1016/s0003-2670(00)81882-6
ISSN1873-4324
AutoresS. Peguin, Pierre R. Coulet, G. Bardeletti,
Tópico(s)Tryptophan and brain disorders
ResumoPyruvate, glutamate pyruvate transaminase (GPT), or glutamate oxaloacetate transaminase (GOT) were determined with a specific enzyme electrode using pyruvate oxidase alone or mixed with oxaloacetate decarboxylase, immobilized on a preactivated membrane. Conditions for immobilization, assays and membrane storage were optimized. For the detection of pyruvate in foodstuffs or biological samples, the detection limit was 1–10−5 M with a linear calibration range of 2–10−5 1–0.1 M. The correlation coefficient with a commercial kit was 0.996. With a two-electrode-based analyzer using pyruvate oxidase immobilized on one electrode and oxaloacetate decarboxylase and pyruvate oxidase co-immobilized and set on the second electrode, GOT and GPT activities were simultaneously determined in < 4 min under the same conditions at optimized substrate concentrations. A linear range of 6–30 000 U 1−1 in the sample, a detection limit of 3 U 1−1, with RSD values of 4.1% and 4.5% and correlation coefficients with the commercial kit of 0.997 and 0.996 for GPT and GOT, respectively, were obtained.
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