Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

H2O2 plays an important role in the lifestyle of Colletotrichum gloeosporioides during interaction with cowpea [Vigna unguiculata (L.) Walp.]

2015; Elsevier BV; Volume: 119; Issue: 8 Linguagem: Inglês

10.1016/j.funbio.2015.05.001

1878-6162

Ygor Raphael Gomes Eloy, Ilka M. Vasconcelos, A. L. H. Barreto, Francisco R. Freire-Filho, José T.A. Oliveira,

Legume Nitrogen Fixing Symbiosis

Plant-fungus interactions usually generate H2O2 in the infected plant tissue. H2O2 has a direct antimicrobial effect and is involved in the cross-linking of cell walls, signaling, induction of gene expression, hypersensitive cell death and induced systemic acquired resistance. This has raised the hypothesis that H2O2 manipulation by pharmacological compounds could alter the lifestyle of Colletotrichum gloeosporioides during interaction with the BR-3-Tracuateua cowpea genotype. The primary leaves of cowpea were excised, infiltrated with salicylic acid (SA), glucose oxidase + glucose (GO/G), catalase (CAT) or diphenyliodonium chloride (DPI), followed by spore inoculation on the adaxial leaf surface. SA or GO/G-treated plantlets showed increased H2O2 accumulation and lipid peroxidation. The fungus used a subcuticular, intramural necrotrophic strategy, and developed secondary hyphae associated with the quick spread and rapid killing of host cells. However, CAT or DPI-treated leaves exhibited decreased H2O2 concentration and lipid peroxidation and the fungus developed intracellular hemibiotrophic infection with vesicles, in addition to primary and secondary hyphal formation. These results suggest that H2O2 plays an important role in the cowpea (C. gloeosporioides) pathosystem given that it affected fungal lifestyle during interaction.