Attachment of human pancreatic tumor cell lines to collagen in vitro.
1981; National Institutes of Health; Volume: 41; Issue: 9 Pt 1 Linguagem: Inglês
Autores
Laurence J. McIntyre, Hynda K. Kleinman, George R. Martin, Young S. Kim,
Tópico(s)Proteoglycans and glycosaminoglycans research
ResumoThe attachment to purified collagens of three cell lines established from human pancreatic carcinomas was investigated. PANC-1 and CAPAN-1 cells attached to type I, III, and IV collagens in the absence of fetal bovine serum. MIA PaCa-2 cells, on the other hand, attached only to type IV collagen. In the case of MIA PaCa-2 cells, the attachment occurs more slowly and to a lesser extent. Increasing concentrations of fetal bovine serum had no effect on the attachment of PANC-1 and CAPAN-1 cells to the collagens. However, the attachment of MIA PaCa-2 cells to all the collagen types was greatly enhanced by 10% fetal bovine serum. This enhancement was shown to be due to the fibronectin present in the serum.
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