Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Farnesol induces the transcriptional accumulation of the Aspergillus nidulans Apoptosis‐Inducing Factor (AIF)‐like mitochondrial oxidoreductase

2008; Wiley; Volume: 70; Issue: 1 Linguagem: Inglês

10.1111/j.1365-2958.2008.06385.x

1365-2958

Marcela Savoldi, Iran Malavazi, Frederico Marianetti Soriani, José Luiz Capellaro, Katsuhiko Kitamoto, Márcia Eliana da Silva Ferreira, Maria Helena S. Goldman, Gustavo H. Goldman,

Photosynthetic Processes and Mechanisms

Summary Farnesol (FOH) is a non‐sterol isoprenoid produced by dephosphorylation of farnesyl pyrophosphate, a catabolite of the cholesterol biosynthetic pathway. These isoprenoids inhibit proliferation and induce apoptosis. It has been shown previously that FOH triggers morphological features characteristic of apoptosis in the filamentous fungus Aspergillus nidulans . Here, we investigate which pathways are influenced through FOH by examining the transcriptional profile of A. nidulans exposed to this isoprenoid. We observed decreased mRNA abundance of several genes involved in RNA processing and modification, transcription, translation, ribosomal structure and biogenesis, amino acid transport and metabolism, and ergosterol biosynthesis. We also observed increased mRNA expression of genes encoding a number of mitochondrial proteins and characterized in detail one of them, the aifA , encoding the Apoptosis‐Inducing Factor (AIF)‐like mitochondrial oxidoreductase. The Δ aifA mutant is more sensitive to FOH (about 8.0% and 0% survival when exposed to 10 and 100 μM FOH respectively) than the wild type (about 97% and 3% survival when exposed to 10 and 100 μM FOH respectively). These results suggest that AifA is possibly important for decreasing the effects of FOH and reactive oxygen species. Furthermore, we showed an involvement of autophagy and protein kinase C in A. nidulans FOH‐induced apoptosis.