Identification of insulin receptor tyrosine residues autophosphorylated in vitro.
1987; Elsevier BV; Volume: 262; Issue: 21 Linguagem: Inglês
10.1016/s0021-9258(18)61100-7
ISSN1083-351X
AutoresHans Törnqvist, Mark Pierce, A. Raymond Frackelton, Raphael A. Nemenoff, Joseph Avruch,
Tópico(s)Pancreatic function and diabetes
ResumoTo identify the autophosphorylation sites on the human insulin receptor (IR), partially purified human IR was incubated in vitro in the presence of insulin and manganese [y3'P]ATP so as to achieve near-maximal activation of the histone 2b kinase activity.Approximately 70% of all @ subunit [32P]phosphotyrosine resides on two tryptic peptide segments identified by microsequencing as IR precursor (Ullrich, A., Bell, J.
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