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In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development.

1991; Springer Nature; Volume: 10; Issue: 8 Linguagem: Inglês

10.1002/j.1460-2075.1991.tb07744.x

1460-2075

S Nishikawa, Moriaki Kusakabe, K. Yoshinaga, Minetaro Ogawa, Shinichi Hayashi, Takahiro Kunisada, Takumi Era, Teruyo Sakakura, Shin-Ichi Nishikawa,

T-cell and B-cell Immunology

Research Article1 August 1991free access In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development. S. Nishikawa S. Nishikawa Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author M. Kusakabe M. Kusakabe Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author K. Yoshinaga K. Yoshinaga Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author M. Ogawa M. Ogawa Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author S. Hayashi S. Hayashi Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author T. Kunisada T. Kunisada Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author T. Era T. Era Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author T. Sakakura T. Sakakura Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author S. Nishikawa S. Nishikawa Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author S. Nishikawa S. Nishikawa Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author M. Kusakabe M. Kusakabe Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author K. Yoshinaga K. Yoshinaga Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author M. Ogawa M. Ogawa Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author S. Hayashi S. Hayashi Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author T. Kunisada T. Kunisada Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author T. Era T. Era Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author T. Sakakura T. Sakakura Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author S. Nishikawa S. Nishikawa Department of Pathology, Kumamoto University Medical School, Japan. Search for more papers by this author Author Information S. Nishikawa1, M. Kusakabe1, K. Yoshinaga1, M. Ogawa1, S. Hayashi1, T. Kunisada1, T. Era1, T. Sakakura1 and S. Nishikawa1 1Department of Pathology, Kumamoto University Medical School, Japan. The EMBO Journal (1991)10:2111-2118https://doi.org/10.1002/j.1460-2075.1991.tb07744.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Previous studies on mice bearing various mutations within the c-kit gene, dominant white spotting (W), indicate the functional role of this tyrosine kinase receptor in the development of melanocytes, germ cells and hematopoietic cells. Despite the availability of mice defective in the c-kit gene and a respectable understanding of the molecular nature of c-kit, however, it is not clear at what stage of gestation c-kit is functionally required for the development of each of these cell lineages. To address this question, we have used a monoclonal anti-c-kit antibody, ACK2, as an antagonistic blocker of c-kit function to interfere with the development of melanocytes during embryonic and postnatal life. ACK2 injected intradermally into pregnant mice entered the embryos where it blocked the proper development of melanocytes. This inhibitory effect was manifested as coat color alteration in the offspring. Furthermore, ACK2 injection also altered the coat color of neonatal and adult mice. Based on the coat color patterns produced by ACK2 administration at various stages before or after birth, the following conclusions are drawn: (i) during mid-gestation, c-kit is functionally required during a restricted period around day 14.5 post-coitum when a sequence of events leading to melanocyte entry into the epidermal layer occurs; (ii) during postnatal life, c-kit is required for melanocyte activation which occurs concomitantly with the hair cycle which continues throughout life after neonatal development of the first hair. Previous ArticleNext Article Volume 10Issue 81 August 1991In this issue RelatedDetailsLoading ...