Mitogenic factor from inbred guinea pigs
1973; Elsevier BV; Volume: 9; Issue: 2 Linguagem: Inglês
10.1016/0008-8749(73)90070-1
ISSN1090-2163
AutoresJeffrey M. Jones, David A. Hart, Alfred Nisonoff,
Tópico(s)Drug Transport and Resistance Mechanisms
ResumoProperties of a mitogenic factor from lymphocytes of inbred guinea pigs were investigated. Experiments in which the antigen was removed from supernatant fractions containing mitogenic factor (MF) indicate conclusively that MF activity does not depend on the presence of antigen. Also, under the conditions tested, MF did not potentiate the response of immune cells to antigen. Highly purified lymphocytes were able to respond to MF, indicating a lack of a requirement for other cell types in the response to MF as well as in its production. Studies of the kinetics of stimulation of nonimmune lymph node cells by MF showed that MF induces DNA synthesis and mitosis starting approximately 24 to 36 hr after the beginning of incubation. Supernatants containing MF stimulate blast transformaion in a subpopulation of lymphocytes; only a small fraction of these transformed cells synthesize DNA and undergo mitosis. About 10% of the cell population was induced by MF to undergo blast transformation. These findings distinguish MF from factors such as lymphocyte-activating material and potentiating factor and provide a basis for further studies of its properties.
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