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Plaque Assay of Nuclear Polyhedrosis Viruses in Cell Culture

1978; American Society for Microbiology; Volume: 36; Issue: 1 Linguagem: Inglês

10.1128/aem.36.1.31-35.1978

1098-5336

Martha Brown, P. Faulkner,

Virus-based gene therapy research

The nuclear polyhedrosis virus of Autographa californica has been titrated in Spodoptera frugiperda cells by the plaque method, using a solid overlay which does not require either the use of modified culture medium or expensive purified agarose or the addition of culture medium as a liquid layer above the solid agarose. This assay is more sensitive than that using a viscous methyl cellulose overlay but less sensitive than the end-point dilution technique. Neither Trichoplusia ni nor Bombyx mori cells were satisfactory as indicators for the assay as described, since they failed to form a stable monolayer. Manduca sexta cells could be utilized for assay of A. californica nuclear polyhedrosis virus, but the sensitivity was lower than with S. frugiperda cells.