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Monoclonal antibodies that detect live salmonellae

1992; American Society for Microbiology; Volume: 58; Issue: 12 Linguagem: Inglês

10.1128/aem.58.12.3868-3872.1992

1098-5336

Ruurd Torensma, M J Visser, Colinda J.M. Aarsman, Miriam J.J.G. Poppelier, Rob Van Beurden, Ad C. Fluit, J. Verhoef,

Escherichia coli research studies

Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were reactive with serogroups A, B, C1, C2, D, E, and K and Salmonella choleraesuis subsp. diarizonae. Each monoclonal antibody proved to be reactive with a distinct serotype. Clinical isolates belonging to these Salmonella serogroups could be detected. Reactivity with non-Salmonella bacteria proved to be minor.