A Study of the Mechanism of Conversion of Galactose to Glucose in the Intact Rat
1963; Elsevier BV; Volume: 238; Issue: 4 Linguagem: Inglês
10.1016/s0021-9258(18)81163-2
ISSN1083-351X
AutoresBernice D. Kohn, Rita C. Hoffmann, Paul M. Kohn,
Tópico(s)Amino Acid Enzymes and Metabolism
ResumoThe conversion of galactose to glucose was studied by administering galactose labeled uniformly with C/sup 14/ and at position 4 with tritium to rats and examining glucose obtained from liver glycogen. The ratio of tritium to C/ sup 14/ in the isolated glucose was unchanged from that in the administered galactose, indicating that if the mechanism of conversion involved the participation of NAD/sup +/ in an oxidation-reduction process, the NAD/sup +/ must have been bound to the enzyme and brought about both the oxidation and the reduction. Other possible mechanisms of conversion are discussed from the viewpoint of binding by the enzyme of reactants in the conversion. (auth)
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