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Quantitation of glucosamine sulfate in plasma by HPLC‐MS/MS after administration of powder for oral solution formulation

2011; Wiley; Volume: 26; Issue: 7 Linguagem: Inglês

10.1002/bmc.1740

1099-0801

Isabela Costa César, Ricardo Martins Duarte Byrro, Fabiana Fernandes de Santana e Silva Cardoso, Iram Moreira Mundim, Leonardo de Souza Teixeira, Kênnia Rocha Rezende, Enikson Pontes da Silva, Sandro Antônio Gomes, Vilmar Alves de Sousa, Ricardo Rodrigues Bonfim, Gérson Antônio Pianetti,

Analytical Chemistry and Chromatography

A rapid method for the quantification of glucosamine in human plasma using high-performance liquid chromatography coupled to tandem mass spectrometry was developed and validated. The sample preparation includes a simple deproteinization step, using D-[1-¹³C] glucosamine hydrochloride as an internal standard. Chromatographic separation was performed on an ACE Ciano column using isocratic elution with acetonitrile and aqueous 2 mM ammonium acetate containing 0.025% formic acid (80:20). Selected reaction monitoring was performed using the transitions m/z 180.1 → m/z 72.1 and m/z 181.0 → m/z 74.6 to quantify glucosamine and internal standard, respectively. The method was validated and proved to be linear, accurate and precise over the range 50-5000 ng/mL of glucosamine. Recovery rates higher than 90% were obtained for both glucosamine and internal standard. No matrix effect was detected in the samples. The validated method was successfully applied to a pharmacokinetic study after oral administration of a powder for oral solution formulation containing glucosamine sulfate.