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Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes.

1990; Springer Nature; Volume: 9; Issue: 3 Linguagem: Inglês

10.1002/j.1460-2075.1990.tb08168.x

1460-2075

D. J. Smith, Martin Burnham, John H. Bull, John E. Hodgson, John M. Ward, Philip Browne, John W. Brown, Barry Barton, Alison J. Earl, G. Turner,

Genomics and Phylogenetic Studies

Research Article1 March 1990free access Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes. D.J. Smith D.J. Smith Department of Microbiology, University of Bristol, UK. Search for more papers by this author M.K. Burnham M.K. Burnham Department of Microbiology, University of Bristol, UK. Search for more papers by this author J.H. Bull J.H. Bull Department of Microbiology, University of Bristol, UK. Search for more papers by this author J.E. Hodgson J.E. Hodgson Department of Microbiology, University of Bristol, UK. Search for more papers by this author J.M. Ward J.M. Ward Department of Microbiology, University of Bristol, UK. Search for more papers by this author P. Browne P. Browne Department of Microbiology, University of Bristol, UK. Search for more papers by this author J. Brown J. Brown Department of Microbiology, University of Bristol, UK. Search for more papers by this author B. Barton B. Barton Department of Microbiology, University of Bristol, UK. Search for more papers by this author A.J. Earl A.J. Earl Department of Microbiology, University of Bristol, UK. Search for more papers by this author G. Turner G. Turner Department of Microbiology, University of Bristol, UK. Search for more papers by this author D.J. Smith D.J. Smith Department of Microbiology, University of Bristol, UK. Search for more papers by this author M.K. Burnham M.K. Burnham Department of Microbiology, University of Bristol, UK. Search for more papers by this author J.H. Bull J.H. Bull Department of Microbiology, University of Bristol, UK. Search for more papers by this author J.E. Hodgson J.E. Hodgson Department of Microbiology, University of Bristol, UK. Search for more papers by this author J.M. Ward J.M. Ward Department of Microbiology, University of Bristol, UK. Search for more papers by this author P. Browne P. Browne Department of Microbiology, University of Bristol, UK. Search for more papers by this author J. Brown J. Brown Department of Microbiology, University of Bristol, UK. Search for more papers by this author B. Barton B. Barton Department of Microbiology, University of Bristol, UK. Search for more papers by this author A.J. Earl A.J. Earl Department of Microbiology, University of Bristol, UK. Search for more papers by this author G. Turner G. Turner Department of Microbiology, University of Bristol, UK. Search for more papers by this author Author Information D.J. Smith1, M.K. Burnham1, J.H. Bull1, J.E. Hodgson1, J.M. Ward1, P. Browne1, J. Brown1, B. Barton1, A.J. Earl1 and G. Turner1 1Department of Microbiology, University of Bristol, UK. The EMBO Journal (1990)9:741-747https://doi.org/10.1002/j.1460-2075.1990.tb08168.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster. Previous ArticleNext Article Volume 9Issue 31 March 1990In this issue RelatedDetailsLoading ...