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Advantages of a modified toluidine blue O stain and bronchoalveolar lavage for the diagnosis of Pneumocystis carinii pneumonia

1985; American Society for Microbiology; Volume: 22; Issue: 5 Linguagem: Inglês

10.1128/jcm.22.5.803-807.1985

1098-660X

L L Gosey, Russell M. Howard, Frank G. Witebsky, Frederick P. Ognibene, Ting‐Shuan Wu, V J Gill, J D MacLowry,

Pneumocystis jirovecii pneumonia detection and treatment

A modified toluidine blue O staining technique for Pneumocystis carinii is described. An easily prepared sulfation reagent made with sulfuric and acetic acids was used. The stain can be employed for bronchoalveolar lavages and lung tissue touch preparations. Most background material was removed by the sulfation reagent, slides were generally easy to read, and time from receipt of a specimen to reporting of results was approximately 1 h. P. carinii cysts were more easily visualized with this stain than in slides stained with modified methylene blue, Gram, Gram-Weigert, and standard toluidine blue O procedures. The importance of certain procedural aspects of subsegmental bronchoalveolar lavage, by which most of the specimens were obtained, is also emphasized.