Evidence for Myosin-like Intermediates in the Hydrolysis of Adenosine Triphosphate by Sperm-Tail Flagella
1972; Elsevier BV; Volume: 247; Issue: 12 Linguagem: Inglês
10.1016/s0021-9258(19)45144-2
ISSN1083-351X
AutoresRichard S. Barclay, Ralph G. Yount,
Tópico(s)Physiological and biochemical adaptations
ResumoAbstract The ATPase of glycerinated sperm flagella from the sea urchin (Strongylocentrotus franciscanus) catalyzes the preferential exchange of water oxygens into the phosphate originating from ATP. This type of exchange, so far known only for myosin-related ATPases, occurred under conditions (60 mm Mg2+, 1 to 10 mm Ca2+) in which no inorganic phosphate-water exchange or exchange into unhydrolyzed ATP was observed. These results suggest that similar intermediates are formed by both skeletal myosin and flagellar ATPase during the cleavage of ATP, in spite of the known large differences in physical and enzymatic properties of the two contractile systems.
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