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In Vitro Reconstitution of T Cell Deficient Mouse Spleen Cells by Co-Cultivation with Human Lymphocytes

1974; American Association of Immunologists; Volume: 112; Issue: 4 Linguagem: Inglês

10.4049/jimmunol.112.4.1613

1550-6606

John J. Farrar,

Organ Transplantation Techniques and Outcomes

Summary The results presented in this report have shown: 1) that human leukocytes are capable of reconstituting the in vitro PFC response of a population of mouse spleen cells known to be deficient in T helper cells; 2) the active cells in the human leukocyte population are resistant to 800 R x-irradiation; and 3) the cells are not removed by carbonyl iron treatment which is known to remove phagocytic cells. The data therefore support the contention that a radioresistant human T lymphocyte is capable of interacting synergistically with mouse B lymphocytes. A subsequent report (15) will present experiments in which double-chambered culture vessels have been used to demonstrate that HPL mediate this xenogeneic “helper” function via a diffusible factor. A number of other questions will also be considered including the antigenic specificity of the active factor.