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Plant histone 2 from wheat germ, a family of histone H2A variants

1979; Elsevier BV; Volume: 578; Issue: 1 Linguagem: Inglês

10.1016/0005-2795(79)90127-2

1878-1454

Jeronimo de ANDRADE RODRIGUES, Wolf F. Brandt, Claus von Holt,

Natural product bioactivities and synthesis

1. The 0.5 M perchloric acid extract prepared from chromatin of wheat germ, Triticum aestivum, contains a group of histones formerly called plant histones. These can be resolved by gel filtration on Bio-Gel P-60 with subsequent CM-cellulose ion-exchange chromatography into five histone fractions containing families of histones H2A and H2B. 2. The partial amino acid sequences of histone H2A variants H2A(1)Triticum, H2A(2)Triticum and H2A(3)Triticum are presented. Extensive sequence homology exists between calf thymus histone H2A and wheat embryo H2A histones. Differences are largely due to conservative amino acid substitutions and in two of the variants, viz. H2A(2) and H2A(3) to N-terminal extensions of the polypeptide chains.