Gelatin- and hydroxyapatite-based cryogels for bone tissue engineering: synthesis, characterization, in vitro and in vivo biocompatibility
2013; Wiley; Volume: 11; Issue: 1 Linguagem: Inglês
10.1002/term.1813
ISSN1932-7005
Autores Tópico(s)3D Printing in Biomedical Research
ResumoJournal of Tissue Engineering and Regenerative MedicineVolume 11, Issue 1 p. 20-33 Research Article Gelatin- and hydroxyapatite-based cryogels for bone tissue engineering: synthesis, characterization, in vitro and in vivo biocompatibility Nevsal Kemençe, Nevsal Kemençe Engineering Faculty, Chemical Engineering Department, Mersin University, 33343 Mezitli, Mersin, TurkeySearch for more papers by this authorNimet Bölgen, Corresponding Author Nimet Bölgen [email protected] Engineering Faculty, Chemical Engineering Department, Mersin University, 33343 Mezitli, Mersin, TurkeyCorrespondence to: N. Bölgen, Engineering Faculty, Chemical Engineering Department, Mersin University, 33343 Mezitli, Mersin, Turkey. E-mail: [email protected] or [email protected]Search for more papers by this author Nevsal Kemençe, Nevsal Kemençe Engineering Faculty, Chemical Engineering Department, Mersin University, 33343 Mezitli, Mersin, TurkeySearch for more papers by this authorNimet Bölgen, Corresponding Author Nimet Bölgen [email protected] Engineering Faculty, Chemical Engineering Department, Mersin University, 33343 Mezitli, Mersin, TurkeyCorrespondence to: N. Bölgen, Engineering Faculty, Chemical Engineering Department, Mersin University, 33343 Mezitli, Mersin, Turkey. E-mail: [email protected] or [email protected]Search for more papers by this author First published: 28 August 2013 https://doi.org/10.1002/term.1813Citations: 47Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract The aim of this study was the synthesis and characterization of gelatin- and hydroxyapatite (osteoconductive component of bone)-based cryogels for tissue-engineering applications. Preliminary in vitro and in vivo biocompatibility tests were conducted. Gelatin- and hydroxyapatite-based cryogels of varying concentrations were synthesized using glutaraldehyde as the crosslinking agent. Chemical structure, pore morphology, pore size distribution, mechanical properties, swelling characteristics and degradation profiles of the synthesized cryogels were demonstrated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mercury porosimetry, a mechanical test device, swelling ratio tests and weight loss measurements, respectively. In vitro cell viability and in vivo biocompatility tests were performed in order to show the performance of the cryogels in the biological environment. Changing the concentrations of gelatin, hydroxyapatite and crosslinker changed the chemical structure, pore size and pore size distribution of the cryogels, which in turn resulted in the ultimate behaviour (mechanical properties, swelling ratio, degradation profile). In vitro cell culture tests showed the viability of the cells. 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