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Characterization of the human papillomavirus E2 protein: evidence of trans-activation and trans-repression in cervical keratinocytes.

1994; Springer Nature; Volume: 13; Issue: 22 Linguagem: Inglês

10.1002/j.1460-2075.1994.tb06880.x

1460-2075

Véronique Bouvard, Alan Storey, D. Pim, Lawrence Banks,

Poxvirus research and outbreaks

Research Article15 November 1994free access Characterization of the human papillomavirus E2 protein: evidence of trans-activation and trans-repression in cervical keratinocytes. V. Bouvard V. Bouvard International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. Search for more papers by this author A. Storey A. Storey International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. Search for more papers by this author D. Pim D. Pim International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. Search for more papers by this author L. Banks L. Banks International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. Search for more papers by this author V. Bouvard V. Bouvard International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. Search for more papers by this author A. Storey A. Storey International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. Search for more papers by this author D. Pim D. Pim International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. Search for more papers by this author L. Banks L. Banks International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. Search for more papers by this author Author Information V. Bouvard1, A. Storey1, D. Pim1 and L. Banks1 1International Centre for Genetic Engineering and Biotechnology, Trieste, Italy. The EMBO Journal (1994)13:5451-5459https://doi.org/10.1002/j.1460-2075.1994.tb06880.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The major regulator of papillomavirus transcription is encoded by the viral E2 gene. The E2 gene has been well characterized in bovine papillomavirus (BPV) where it encodes at least three different polypeptides which differentially affect viral gene expression. In human papillomaviruses (HPVs) the E2 gene product is much less well characterized. In this study we have analysed the mechanism of action of the HPV-16, HPV-18 and BPV-1 E2 proteins in cervical keratinocytes. We show that the full length HPV E2 protein acts as a potent transcriptional activator of viral gene expression in both normal and immortalized keratinocytes. In contrast, the BPV-1 E2 protein produces transcriptional repression under identical conditions. A cDNA encoding the C-terminal half of the HPV-16 E2 protein in these assays weakly repressed viral gene expression. Further, co-transfection of this cDNA with the full length clone progressively abolishes the activation in trans by the full length HPV E2 protein. Gel retardation assays have defined a number of protein complexes between the long and short forms of E2 but with no evidence for preferential DNA binding. These results define two distinct activities for the HPV-16 E2 protein, indicate functional differences with the BPV E2 protein and suggest that splicing of the HPV E2 mRNA is a critical mechanism for controlling viral gene expression. Previous ArticleNext Article Volume 13Issue 221 November 1994In this issue RelatedDetailsLoading ...