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Corosolic Acid Exhibits Anti-angiogenic and Anti-lymphangiogenic Effects on In Vitro Endothelial Cells and on an In Vivo CT-26 Colon Carcinoma Animal Model

2015; Wiley; Volume: 29; Issue: 5 Linguagem: Inglês

10.1002/ptr.5306

1099-1573

Ki Hyun Yoo, Jong-Hwa Park, Dae Young Lee, Jeon Hwang‐Bo, Nam In Baek, In Sik Chung,

Inflammation biomarkers and pathways

Phytotherapy ResearchVolume 29, Issue 5 p. 714-723 Research Article Corosolic Acid Exhibits Anti-angiogenic and Anti-lymphangiogenic Effects on In Vitro Endothelial Cells and on an In Vivo CT-26 Colon Carcinoma Animal Model Ki Hyun Yoo, Ki Hyun Yoo Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaThese authors contributed equally to this work.Search for more papers by this authorJong-Hwa Park, Jong-Hwa Park Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaThese authors contributed equally to this work.Search for more papers by this authorDae Young Lee, Dae Young Lee Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaSearch for more papers by this authorJeon Hwang-Bo, Jeon Hwang-Bo Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaSearch for more papers by this authorNam In Baek, Nam In Baek Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaSearch for more papers by this authorIn Sik Chung, Corresponding Author In Sik Chung Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 Korea Correspondence to: In Sik Chung, Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea. E-mail: ischung@khu.ac.krSearch for more papers by this author Ki Hyun Yoo, Ki Hyun Yoo Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaThese authors contributed equally to this work.Search for more papers by this authorJong-Hwa Park, Jong-Hwa Park Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaThese authors contributed equally to this work.Search for more papers by this authorDae Young Lee, Dae Young Lee Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaSearch for more papers by this authorJeon Hwang-Bo, Jeon Hwang-Bo Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaSearch for more papers by this authorNam In Baek, Nam In Baek Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 KoreaSearch for more papers by this authorIn Sik Chung, Corresponding Author In Sik Chung Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701 Korea Correspondence to: In Sik Chung, Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea. E-mail: ischung@khu.ac.krSearch for more papers by this author First published: 02 February 2015 https://doi.org/10.1002/ptr.5306Citations: 21 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract We describe the anti-angiogenic and anti-lymphangiogenic effects of corosolic acid, a pentacyclic triterpenoid isolated from Cornus kousa Burg. A mouse colon carcinoma CT-26 animal model was employed to determine the in vivo anti-angiogenic and anti-lymphangiogenic effects of corosolic acid. Corosolic acid induced apoptosis in CT-26 cells, mediated by the activation of caspase-3. In addition, it reduced the final tumor volume and the blood and lymphatic vessel densities of tumors, indicating that it suppresses in vivo angiogenesis and lymphangiogenesis. Corosolic acid inhibited the proliferation and tube formation of human umbilical vein endothelial cells and human dermal lymphatic microvascular endothelial cells. In addition, corosolic acid decreased the proliferation and migration of human umbilical vein endothelial cells stimulated by angiopoietin-1. Pretreatment with corosolic acid decreased the phosphorylation of focal adhesion kinase (FAK) and ERK1/2, suggesting that corosolic acid contains anti-angiogenic activity that can suppress FAK signaling induced by angiopoietin-1. Copyright © 2015 John Wiley & Sons, Ltd. Citing Literature Supporting Information Supplementary data 1. Annexin V analysis of corosolic acid-treated CT-26 cells. CT-26 cells were seeded onto culture chamber slide glasses (2 cm2; Thermo Fisher Scientific Inc.) at a density of 2 × 104 cells/slide and incubated for 24 h. After treatment with serum-free Dulbecco's modified Eagle's medium containing 25 µm corosolic acid for 12 and 24 h, cells were labeled with the annexin V-FITC conjugate (Santa Cruz Biotech.) according to the manufacturer's instructions. Cells were then stained with 0.2 µm propidium iodide (Sigma) for 20 min on ice in the dark. fluorescein isothiocyanate (FITC) and propidium iodide fluorescences were imaged under ×40 objective magnification using a confocal laser scanning microscope (LSM 510 Meta, Carl Zeiss, Germany) (bar = 20 µm). CA means corosolic acid. DIC represents the differential inference contrast. Filename Description ptr5306-sup-0001-documentS1.tifTIFF image, 7.8 MB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume29, Issue5May 2015Pages 714-723 RelatedInformation