Purification of four forms of the beta gamma subunit complex of G proteins containing different gamma subunits.
1993; Elsevier BV; Volume: 268; Issue: 27 Linguagem: Inglês
10.1016/s0021-9258(20)80755-8
ISSN1083-351X
AutoresTaku Asano, Rika Morishita, Tomoki Matsuda, Yoshitaka Fukada, T. Yoshizawa, Keisuke Kato,
Tópico(s)Enzyme Structure and Function
ResumoTo investigate the physiological significance of the diversity of y subunits of G proteins, we purified four forms of By of G proteins from bovine brain (By-Bl, By-B2, By-B3), and spleen (By-Sl) by the sequential chromatography on columns of DEAE-Sephacel, Ultrogel AcA 34, heptylamine-Sepharose, phenyl-BPW, and DEAE-5PW.Electrophoretic analyses showed that each By mainly contained the 36-kDa j3 and a distinct but homogeneous y.These By complexes were subjected directly to proteolytic digestion and subsequent amino acid sequence analyses of their fragments.It was revealed that By-B1, -B2, and -B3 were identical to
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