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Circular Herpesvirus sylvilagus DNA in spleen cells of experimentally infected cottontail rabbits

1984; American Society for Microbiology; Volume: 52; Issue: 2 Linguagem: Inglês

10.1128/jvi.52.2.711-714.1984

1098-5514

Peter G. Medveczky, William Kramp, J L Sullivan,

Viral-associated cancers and disorders

Cottontail rabbits (Sylvilagus floridanus) were infected with Herpesvirus sylvilagus, and spleen cells were analyzed for the presence of virus-specific, covalently closed circular, and linear DNA molecules by a simple electrophoretic technique, followed by transfer to nitrocellulose filters and hybridization with cloned viral DNA (Gardella et al., J. Virol. 50:248-254, 1984). Approximately 0.2 copies per cell of circular DNA and 0.2 copies per cell of linear DNA were detected by hybridization with a cloned viral DNA fragment. The size of the viral DNA was estimated at ca. 158 kilobase pairs. Restriction endonuclease patterns suggested structural similarities to cottontail herpesvirus DNA.