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Paternal alcohol exposure in mice alters brain NGF and BDNF and increases ethanol-elicited preference in male offspring

2015; Wiley; Volume: 21; Issue: 4 Linguagem: Inglês

10.1111/adb.12255

1369-1600

Mauro Ceccanti, Roberto Coccurello, Valentina Carito, Stefania Ciafrè, Giampiero Ferraguti, Giacomo Giacovazzo, Rosanna Mancinelli, Paola Tirassa, George N. Chaldakov, Esterina Pascale, Marco Ceccanti, Claudia Codazzo, Marco Fiore,

Birth, Development, and Health

Addiction BiologyVolume 21, Issue 4 p. 776-787 PRECLINICAL STUDY Paternal alcohol exposure in mice alters brain NGF and BDNF and increases ethanol-elicited preference in male offspring Mauro Ceccanti, Mauro Ceccanti Centro Riferimento Alcologico Regione Lazio, Sapienza University of Rome, ItalyThe authors contributed equally to this article.Search for more papers by this authorRoberto Coccurello, Roberto Coccurello Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalyThe authors contributed equally to this article.Search for more papers by this authorValentina Carito, Valentina Carito Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalySearch for more papers by this authorStefania Ciafrè, Stefania Ciafrè Institute of Translational Pharmacology (IFT), National Research Council of Italy (C.N.R.), ItalySearch for more papers by this authorGiampiero Ferraguti, Giampiero Ferraguti Department of Cellular Biotechnologies and Hematology, Sapienza University of Rome, ItalySearch for more papers by this authorGiacomo Giacovazzo, Giacomo Giacovazzo Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalySearch for more papers by this authorRosanna Mancinelli, Rosanna Mancinelli Centro Nazionale Sostanze Chimiche, Instituto Superiore di Sanità, Rome, ItalySearch for more papers by this authorPaola Tirassa, Paola Tirassa Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalySearch for more papers by this authorGeorge N. Chaldakov, George N. Chaldakov Laboratory of Cell Biology, Medical University, BulgariaSearch for more papers by this authorEsterina Pascale, Esterina Pascale Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, ItalySearch for more papers by this authorMarco Ceccanti, Marco Ceccanti Department of Neurology and Psychiatry, Sapienza University of Rome, ItalySearch for more papers by this authorClaudia Codazzo, Claudia Codazzo Centro Riferimento Alcologico Regione Lazio, Sapienza University of Rome, ItalySearch for more papers by this authorMarco Fiore, Corresponding Author Marco Fiore Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalyCorrespondence to: Marco Fiore, Institute of Cell Biology and Neurobiology (IBCN)-CNR, Via del Fosso di Fiorano 64, Roma 00143, Italy. E-mail: [email protected]Search for more papers by this author Mauro Ceccanti, Mauro Ceccanti Centro Riferimento Alcologico Regione Lazio, Sapienza University of Rome, ItalyThe authors contributed equally to this article.Search for more papers by this authorRoberto Coccurello, Roberto Coccurello Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalyThe authors contributed equally to this article.Search for more papers by this authorValentina Carito, Valentina Carito Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalySearch for more papers by this authorStefania Ciafrè, Stefania Ciafrè Institute of Translational Pharmacology (IFT), National Research Council of Italy (C.N.R.), ItalySearch for more papers by this authorGiampiero Ferraguti, Giampiero Ferraguti Department of Cellular Biotechnologies and Hematology, Sapienza University of Rome, ItalySearch for more papers by this authorGiacomo Giacovazzo, Giacomo Giacovazzo Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalySearch for more papers by this authorRosanna Mancinelli, Rosanna Mancinelli Centro Nazionale Sostanze Chimiche, Instituto Superiore di Sanità, Rome, ItalySearch for more papers by this authorPaola Tirassa, Paola Tirassa Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalySearch for more papers by this authorGeorge N. Chaldakov, George N. Chaldakov Laboratory of Cell Biology, Medical University, BulgariaSearch for more papers by this authorEsterina Pascale, Esterina Pascale Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, ItalySearch for more papers by this authorMarco Ceccanti, Marco Ceccanti Department of Neurology and Psychiatry, Sapienza University of Rome, ItalySearch for more papers by this authorClaudia Codazzo, Claudia Codazzo Centro Riferimento Alcologico Regione Lazio, Sapienza University of Rome, ItalySearch for more papers by this authorMarco Fiore, Corresponding Author Marco Fiore Institute of Cell Biology and Neurobiology (IBCN)/IRCCS S. Lucia Foundation, ItalyCorrespondence to: Marco Fiore, Institute of Cell Biology and Neurobiology (IBCN)-CNR, Via del Fosso di Fiorano 64, Roma 00143, Italy. E-mail: [email protected]Search for more papers by this author First published: 04 May 2015 https://doi.org/10.1111/adb.12255Citations: 38 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Ethanol (EtOH) exposure during pregnancy induces cognitive and physiological deficits in the offspring. However, the role of paternal alcohol exposure (PAE) on offspring EtOH sensitivity and neurotrophins has not received much attention. The present study examined whether PAE may disrupt nerve growth factor (NGF) and/or brain-derived neurotrophic factor (BDNF) and affect EtOH preference/rewarding properties in the male offspring. CD1 sire mice were chronically addicted for EtOH or administered with sucrose. Their male offsprings when adult were assessed for EtOH preference by a conditioned place preference paradigm. NGF and BDNF, their receptors (p75NTR, TrkA and TrkB), dopamine active transporter (DAT), dopamine receptors D1 and D2, pro-NGF and pro-BDNF were also evaluated in brain areas. PAE affected NGF levels in frontal cortex, striatum, olfactory lobes, hippocampus and hypothalamus. BDNF alterations in frontal cortex, striatum and olfactory lobes were found. PAE induced a higher susceptibility to the EtOH rewarding effects mostly evident at the lower concentration (0.5 g/kg) that was ineffective in non-PAE offsprings. Moreover, higher ethanol concentrations (1.5 g/kg) produced an aversive response in PAE animals and a significant preference in non-PAE offspring. PAE affected also TrkA in the hippocampus and p75NTR in the frontal cortex. DAT was affected in the olfactory lobes in PAE animals treated with 0.5 g/kg of ethanol while no differences were found on D1/D2 receptors and for pro-NGF or pro-BDNF. In conclusion, this study shows that: PAE affects NGF and BDNF expression in the mouse brain; PAE may induce ethanol intake preference in the male offspring. Citing Literature Volume21, Issue4July 2016Pages 776-787 RelatedInformation