Produção Nacional
Revisado por pares
A Stability-Indicating HPLC-DAD Method for Determination of Ferulic Acid into Microparticles: Development, Validation, Forced Degradation, and Encapsulation Efficiency
2015; Hindawi Publishing Corporation; Volume: 2015; Linguagem: Inglês
10.1155/2015/286812
ISSN2090-8865
AutoresJéssica Mendes Nadal, Maria da Graça T. Toledo, Yasmine Mendes Pupo, Josiane Padilha de Paula, Paulo Vítor Farago, Sandra Maria Warumby Zanin,
Tópico(s)Traditional Chinese Medicine Analysis
ResumoA simple stability-indicating HPLC-DAD method was validated for the determination of ferulic acid (FA) in polymeric microparticles. Chromatographic conditions consisted of a RP C 18 column (250 mm × 4.60 mm, 5 μ m, 110 Å) using a mixture of methanol and water pH 3.0 (48 : 52 v/v) as mobile phase at a flow rate of 1.0 mL/min with UV detection at 320 nm. The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of quantification, limit of detection, accuracy, precision, and robustness provided suitable results regarding all parameters investigated. The calibration curve was linear in the concentration range of 10.0–70.0 μ g/mL with a correlation coefficient >0.999. Precision (intraday and interday) was demonstrated by a relative standard deviation lower than 2.0%. Accuracy was assessed by the recovery test of FA from polymeric microparticles (99.02% to 100.73%). Specificity showed no interference from the components of polymeric microparticles or from the degradation products derived from acidic, basic, and photolytic conditions. In conclusion, the method is suitable to be applied to assay FA as bulk drug and into polymeric microparticles and can be used for studying its stability and degradation kinetics.
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