Characterization of the antibody response to type 3 pneumococcal polysaccharide at the cellular level. I. Dose-response studies and the effect of prior immunization on the magnitude of the antibody response.
1971; National Institutes of Health; Volume: 20; Issue: 4 Linguagem: Inglês
Autores
Phillip J. Baker, Philip W. Stashak, Diana F. Amsbaugh, Benjamin Prescott,
Tópico(s)Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
ResumoThe cytokinetics of the antibody to Type III pneumococcal polysaccharide (SSS-III) were characterized by an immuno-plaque procedure using erythrocytes sensitized with SSS-III. Prior immunization, irrespective of the doses employed, did not result in the development of immunological memory; instead, low-dose paralysis was produced in mice previously immunized with all doses of SSS-III. Dose-response studies revealed that within a given dose range, there was a direct relationship between the immunizing dose and the magnitude of the antibody response obtained. The dose-response curve for SSS-III showed a single optimal dose for immunization; doses only slightly in excess of the optimal dose produced a significant reduction in the magnitude of the antibody response. The implications of these findings, with respect to the development of paralysis to SSS-III, are discussed.
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