Blood clotting factor IX. Loss of activity after cleavage of sialic acid residues.
1984; Elsevier BV; Volume: 259; Issue: 6 Linguagem: Inglês
10.1016/s0021-9258(17)43103-6
ISSN1083-351X
AutoresStephen I. Chavin, Stanisław Weidner,
Tópico(s)Protein purification and stability
ResumoEnzymatic cleavage of sialic acid from human blood clotting factor IX results in a loss of factor M clotting activity.The loss of clotting activity and the rate of release of sialic acid follow the same time courses.Control experiments have ruled out several explanations for the loss of factor IX activity: proteolytic degradation, inhibitory effects of free sialic acid, and non- specific inhibition of the clotting assays.Furthermore, no inhibition was seen when similar enzymatic cleavage was carried out on factor X and factor VIII. Therefore, we suggest that the loss of factor IX activity is the direct result of cleavage of sialic acid from the protein.Most of the inhibition appeared to be an effect on the activity of factor IX, itself, and thus far, little or no effect has been shown on the activation of factor IX to IX,.The structural basis for this unusual effect of sialic acid on protein function currently is being investigated.
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