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O‐specific polysaccharide of Hafnia alvei lipopolysaccharide isolated from strain 1211

1991; Wiley; Volume: 200; Issue: 2 Linguagem: Inglês

10.1111/j.1432-1033.1991.tb16198.x

1432-1033

Ewa Katzenellenbogen, Elżbieta Romanowska, Ursula Dąbrowski, J. Dąbrowski,

Probiotics and Fermented Foods

The Hafnia alvei strain 1211 O‐specific polysaccharide is composed of 3‐amino‐ N ‐( d ‐3′‐hydroxybutyryl)‐3,6‐dideoxy‐ d ‐galactose, N ‐acetyl‐ d ‐galactosamine, N ‐acetyl‐ d ‐glucosamine and d ‐glucose (1:1:2:2). On the basis of sugar and methylation analyses, Smith degradation, and one‐ and two‐dimensional 1 H‐ and 13 C‐NMR spectroscopy, the polysaccharide was shown to be an O ‐acetylated polymer of the repeating hexasaccharide unit, → 2 d (4‐ O Ac)Fuc p 3NAcylβ1 → 6 d Glc p NAcα1 → ( d Glc p β1 → 3)4 d Gal p NAcα1 → 3 d Glc p NAcβ1 → 2 d Glc p β1 →, where d Fuc p 3NAcyl = 3‐amino‐ N ‐( d ‐3′‐hydroxybutyryl)‐3,6‐dideoxy‐ d ‐galactopyranose. The O‐specific polysaccharide showed some microheterogeneity due to incomplete substitution by terminal glucose.