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BIBTEX RIS

Occurrence of an Affinity Site apart from the Active Site on the Raw-Starch-Digesting but Non-Raw-Starch-Adsorbable Bacillus subtilis 65 α-Amylase

1990; American Society for Microbiology; Volume: 56; Issue: 8 Linguagem: Inglês

10.1128/aem.56.8.2584-2586.1990

ISSN

1098-5336

Autores

Shinsaku Hayashida, Yuji Teramoto, Takehiro Inoue, Shinji Mitsuiki,

Tópico(s)

Microbial Metabolites in Food Biotechnology

Resumo

α-Cyclodextrin specifically inhibited raw starch digestion by Bacillus subtilis 65 α-amylase. The raw starch digestibility and α-cyclodextrin-Sepharose 6B adsorbability of this α-amylase were simultaneously lost when the specific domain corresponding to the affinity site essential for raw starch digestion was deleted by proteolysis. Occurrence of the affinity site on raw-starch-digesting enzymes was proven also with bacterial amylase.

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