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Rapid diagnostics: the detection of neuraminidase activity as a technology for high-specificity targets

2001; Royal Society; Volume: 356; Issue: 1416 Linguagem: Inglês

10.1098/rstb.2001.10006

1471-2970

Craig Shimasaki, Komandoor E. Achyuthan, J. A. Hansjergen, James R. Appleman,

Advanced Biosensing Techniques and Applications

Restricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article 2001Rapid diagnostics: the detection of neuraminidase activity as a technology for high-specificity targetsPhil. Trans. R. Soc. Lond. B3561925–1931http://doi.org/10.1098/rstb.2001.1006SectionRestricted accessRapid diagnostics: the detection of neuraminidase activity as a technology for high-specificity targets Published:29 December 2001https://doi.org/10.1098/rstb.2001.1006AbstractThe accurate detection of influenza by clinical symptoms is challenging since multiple pathogenic viruses and bacteria mimic similar symptoms in a patient. With new and more effective influenza therapeutics available, there is a growing need for highly accurate and rapid diagnosis of influenza, particularly when the window of opportunity for proper treatment is measured in hours. A parallel technology, which is also used in the treatment of influenza, was developed for the rapid diagnosis of influenza by exploiting the enzymatic activity of influenza neuraminidase. This technology, which is called Pathozyme, offers the high specificity inherent from the conservation of the neuraminidase active site. The ZstatFlu test uses a small molecule derivative of sialic acid chemically coupled to a reporter group together with simple point–of–care reagents for directly detecting influenza from a patient specimen with high specificity. A second–generation platform technology using this neuraminidase detection system coupled with a more sensitive chemiluminescent reporter has been developed and formatted for reading on high–speed instant film. This modification resulted in a platform technology many–fold more sensitive than the former while maintaining its inherent high specificity. Preliminary data from a prototype tested during the mild 2000–2001 influenza season demonstrated that an optimized chemiluminescent test system could approach the accuracy of 14 day viral culture in a convenient 10–20 min test. This platform technology is currently being explored for the rapid detection of other pathogenic organisms where sensitivity, specificity and speed are essential in a point–of–care setting. Previous ArticleNext Article VIEW FULL TEXT DOWNLOAD PDF FiguresRelatedReferencesDetailsCited by Chang H, Mei Y, Li Y and Shang L (2022) An AIE and ESIPT based neuraminidase fluorescent probe for influenza virus detection and imaging, Talanta, 10.1016/j.talanta.2022.123583, 247, (123583), Online publication date: 1-Sep-2022. Yuan L, Zhao Y and Sun X (2020) Sialidase substrates for Sialdiase assays - activity, specificity, quantification and inhibition, Glycoconjugate Journal, 10.1007/s10719-020-09940-0, 37:5, (513-531), Online publication date: 1-Oct-2020. Liu D, Cui X, Dhawane A, Chivukula V and Iyer S (2019) Fluorescent sialic derivatives for the specific detection of influenza viruses, Bioorganic & Medicinal Chemistry Letters, 10.1016/j.bmcl.2019.126773, 29:24, (126773), Online publication date: 1-Dec-2019. Mubarok A, Mani V, Huang C, Chang P and Huang S (2017) Label-free electrochemical detection of neuraminidase activity: A facile whole blood diagnostic probe for infectious diseases, Sensors and Actuators B: Chemical, 10.1016/j.snb.2017.06.061, 252, (641-648), Online publication date: 1-Nov-2017. Turgeon N, Toulouse M, Ho J, Li D and Duchaine C (2017) Neuraminidase as an enzymatic marker for detecting airborne Influenza virus and other viruses , Canadian Journal of Microbiology, 10.1139/cjm-2016-0450, 63:2, (119-128), Online publication date: 1-Feb-2017. Cui X, Das A, Dhawane A, Sweeney J, Zhang X, Chivukula V and Iyer S (2017) Highly specific and rapid glycan based amperometric detection of influenza viruses, Chemical Science, 10.1039/C6SC03720H, 8:5, (3628-3634) Zhang X, Dhawane A, Sweeney J, He Y, Vasireddi M and Iyer S (2015) Electrochemical Assay to Detect Influenza Viruses and Measure Drug Susceptibility, Angewandte Chemie International Edition, 10.1002/anie.201412164, 54:20, (5929-5932), Online publication date: 11-May-2015. Zhang X, Dhawane A, Sweeney J, He Y, Vasireddi M and Iyer S (2015) Electrochemical Assay to Detect Influenza Viruses and Measure Drug Susceptibility, Angewandte Chemie, 10.1002/ange.201412164, 127:20, (6027-6030), Online publication date: 11-May-2015. Harmon H and Oliver A (2014) Optical biodetection using receptors and enzymes (porphyrin-incorporated) Biological Identification, 10.1533/9780857099167.3.253, (253-280), . 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Kamikawa T, Mikolajczyk M, Kennedy M, Zhong L, Zhang P, Setterington E, Scott D and Alocilja E Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance, IEEE Transactions on Nanotechnology, 10.1109/TNANO.2011.2157936, 11:1, (88-96) Turgeon N, McNicoll F, Toulouse M, Liav A, Barbeau J, Ho J, Grund C and Duchaine C (2011) Neuraminidase Activity as a Potential Enzymatic Marker for Rapid Detection of Airborne Viruses, Aerosol Science and Technology, 10.1080/02786826.2010.530624, 45:2, (183-195), Online publication date: 1-Feb-2011. Amano Y and Cheng Q (2004) Detection of influenza virus: traditional approaches and development of biosensors, Analytical and Bioanalytical Chemistry, 10.1007/s00216-004-2927-0, 381:1, (156-164), Online publication date: 1-Jan-2005. Nayak D and Reichl U (2004) Neuraminidase activity assays for monitoring MDCK cell culture derived influenza virus, Journal of Virological Methods, 10.1016/j.jviromet.2004.07.005, 122:1, (9-15), Online publication date: 1-Dec-2004. Achyuthan K (2004) Fluorescent Assays To Quantitate Enzymatic Activities Yielding as End Product an Aqueous-Insoluble Indigo-Blue Dye, Langmuir, 10.1021/la035974u, 20:6, (2424-2428), Online publication date: 1-Mar-2004. Achyuthan K, Pence L, Appleman J and Shimasaki C (2003) ZstatFlu®-II test: a chemiluminescent neuraminidase assay for influenza viral diagnostics, Luminescence, 10.1002/bio.714, 18:3, (131-139), Online publication date: 1-May-2003. Achyuthan K, Pence L, Mantell D, Nangeroni P, Mauchan D, Aitken W, Appleman J and Shimasaki C (2003) Engineering a chemical implementation device and an imaging device for detecting chemiluminescence with a Polaroid™ high-speed detector film: application to influenza diagnostics with the ZstatFlu®-II test, Luminescence, 10.1002/bio.709, 18:2, (79-89), Online publication date: 1-Mar-2003. Laver G and Webster R (2001) Introduction, Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 356:1416, (1813-1815), Online publication date: 29-Dec-2001. This Issue29 December 2001Volume 356Issue 1416Discussion Meeting Issue ‘The origin and control of pandemic influenza’ organized by G. Laver and R. G. Webster Article InformationDOI:https://doi.org/10.1098/rstb.2001.1006PubMed:11779393Published by:Royal SocietyPrint ISSN:0962-8436Online ISSN:1471-2970History: Published online29/12/2001Published in print29/12/2001 License: Citations and impact Keywordsdisease managementchemiluminescencehigh–specificity targetinfluenza; neuraminidaserapid diagnostics