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Inhibition of transcription factors belonging to the rel/NF-kappa B family by a transdominant negative mutant.

1991; Springer Nature; Volume: 10; Issue: 7 Linguagem: Inglês

10.1002/j.1460-2075.1991.tb07708.x

1460-2075

F. Logeat, Nicole Israël, Rosa Ten, Volker Blank, O. Le Bail, Philippe Kourilsky, Alain Israël,

Cytokine Signaling Pathways and Interactions

Research Article1 July 1991free access Inhibition of transcription factors belonging to the rel/NF-kappa B family by a transdominant negative mutant. F. Logeat F. Logeat Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author N. Israël N. Israël Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author R. Ten R. Ten Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author V. Blank V. Blank Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author O. Le Bail O. Le Bail Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author P. Kourilsky P. Kourilsky Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author A. Israël A. Israël Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author F. Logeat F. Logeat Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author N. Israël N. Israël Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author R. Ten R. Ten Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author V. Blank V. Blank Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author O. Le Bail O. Le Bail Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author P. Kourilsky P. Kourilsky Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author A. Israël A. Israël Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. Search for more papers by this author Author Information F. Logeat1, N. Israël1, R. Ten1, V. Blank1, O. Le Bail1, P. Kourilsky1 and A. Israël1 1Unité de Biologie Moléculaire du Gène, U.277 Inserm-U.A. 535 CNRS, Paris, France. The EMBO Journal (1991)10:1827-1832https://doi.org/10.1002/j.1460-2075.1991.tb07708.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The KBF1 factor, which binds to the enhancer A located in the promoter of the mouse MHC class I gene H-2Kb, is indistinguishable from the p50 DNA binding subunit of the transcription factor NF-kappa B, which regulates a series of genes involved in immune and inflammatory responses. The KBF1/p50 factor binds as a homodimer but can also form heterodimers with the products of other members of the same family, like the c-rel and v-rel (proto)oncogenes. The dimerization domain of KBF1/p50 is contained between amino acids 201 and 367. A mutant of KBF1/p50 (delta SP), unable to bind to DNA but able to form homo- or heterodimers, has been constructed. This protein reduces or abolishes in vitro the DNA binding activity of wild-type proteins of the same family (KBF1/p50, c- and v-rel). This mutant also functions in vivo as a trans-acting dominant negative regulator: the transcriptional inducibility of the HIV long terminal repeat (which contains two potential NF-kappa B binding sites) by phorbol ester (PMA) is inhibited when it is co-transfected into CD4+ T cells with the delta SP mutant. Similarly the basal as well as TNF or IL1-induced activity of the MHC class I H-2Kb promoter can be inhibited by this mutant in two different cell lines. These results constitute the first formal demonstration that these genes are regulated by members of the rel/NF-kappa B family. Previous ArticleNext Article Volume 10Issue 71 July 1991In this issue RelatedDetailsLoading ...