A continuous peptide epitope reacting with pandemic influenza AH1N1 predicted by bioinformatic approaches
2015; Wiley; Volume: 28; Issue: 9 Linguagem: Inglês
10.1002/jmr.2470
ISSN1099-1352
AutoresJonathan Pablo Carrillo-Vazquez, José Correa‐Basurto, Jazmín García‐Machorro, Rafael Campos-Rodrı́guez, Violaine Moreau, Jorge L. Rosas‐Trigueros, César A. Reyes‐López, Marlon Rojas‐López, Absalom Zamorano‐Carrillo,
Tópico(s)Machine Learning in Bioinformatics
ResumoJournal of Molecular RecognitionVolume 28, Issue 9 p. 553-564 Research article A continuous peptide epitope reacting with pandemic influenza AH1N1 predicted by bioinformatic approaches Jonathan P. Carrillo-Vazquez, Jonathan P. Carrillo-Vazquez Laboratorio de Bioquímica y Biofísica Computacional, Doctorado en Biotecnología ENMH—IPN, Mexico, D.F., MexicoSearch for more papers by this authorJosé Correa-Basurto, José Correa-Basurto Laboratorio de Modelado Molecular y Diseño de Fármacos, Escuela Superior de Medicina—IPN, Mexico, D.F., MexicoSearch for more papers by this authorJazmin García-Machorro, Jazmin García-Machorro Laboratorio de Medicina de Conservación, Escuela Superior de Medicina—IPN, Mexico, D.F., MexicoSearch for more papers by this authorRafael Campos-Rodríguez, Rafael Campos-Rodríguez Laboratorio de Inmunología Molecular, Escuela Superior de Medicina—IPN, Mexico, D.F., MexicoSearch for more papers by this authorViolaine Moreau, Violaine Moreau CNRS FRE 3009, SysDiag, CAP DELTA, Montpellier, FranceSearch for more papers by this authorJorge L. Rosas-Trigueros, Jorge L. Rosas-Trigueros Laboratorio Transdisciplinario de Investigación en Sistemas Evolutivos SEPI-ESCOM-IPN, Mexico, D.F., MexicoSearch for more papers by this authorCesar A. Reyes-López, Cesar A. Reyes-López Laboratorio de Bioquímica y Biofísica Computacional, Doctorado en Biotecnología ENMH—IPN, Mexico, D.F., MexicoSearch for more papers by this authorMarlon Rojas-López, Marlon Rojas-López Centro de Investigación en Biotecnología Aplicada—IPN, Tlaxcala, MexicoSearch for more papers by this authorAbsalom Zamorano-Carrillo, Corresponding Author Absalom Zamorano-Carrillo Laboratorio de Bioquímica y Biofísica Computacional, Doctorado en Biotecnología ENMH—IPN, Mexico, D.F., Mexico Correspondence to: A. Zamorano-Carrillo, Laboratorio de Bioquímica y Biofísica Computacional, Doctorado en Biotecnología, ENMH—IPN, Mexico, D.F., Mexico. E-mail: absalomz2002@gmail.comSearch for more papers by this author Jonathan P. Carrillo-Vazquez, Jonathan P. Carrillo-Vazquez Laboratorio de Bioquímica y Biofísica Computacional, Doctorado en Biotecnología ENMH—IPN, Mexico, D.F., MexicoSearch for more papers by this authorJosé Correa-Basurto, José Correa-Basurto Laboratorio de Modelado Molecular y Diseño de Fármacos, Escuela Superior de Medicina—IPN, Mexico, D.F., MexicoSearch for more papers by this authorJazmin García-Machorro, Jazmin García-Machorro Laboratorio de Medicina de Conservación, Escuela Superior de Medicina—IPN, Mexico, D.F., MexicoSearch for more papers by this authorRafael Campos-Rodríguez, Rafael Campos-Rodríguez Laboratorio de Inmunología Molecular, Escuela Superior de Medicina—IPN, Mexico, D.F., MexicoSearch for more papers by this authorViolaine Moreau, Violaine Moreau CNRS FRE 3009, SysDiag, CAP DELTA, Montpellier, FranceSearch for more papers by this authorJorge L. Rosas-Trigueros, Jorge L. Rosas-Trigueros Laboratorio Transdisciplinario de Investigación en Sistemas Evolutivos SEPI-ESCOM-IPN, Mexico, D.F., MexicoSearch for more papers by this authorCesar A. Reyes-López, Cesar A. Reyes-López Laboratorio de Bioquímica y Biofísica Computacional, Doctorado en Biotecnología ENMH—IPN, Mexico, D.F., MexicoSearch for more papers by this authorMarlon Rojas-López, Marlon Rojas-López Centro de Investigación en Biotecnología Aplicada—IPN, Tlaxcala, MexicoSearch for more papers by this authorAbsalom Zamorano-Carrillo, Corresponding Author Absalom Zamorano-Carrillo Laboratorio de Bioquímica y Biofísica Computacional, Doctorado en Biotecnología ENMH—IPN, Mexico, D.F., Mexico Correspondence to: A. Zamorano-Carrillo, Laboratorio de Bioquímica y Biofísica Computacional, Doctorado en Biotecnología, ENMH—IPN, Mexico, D.F., Mexico. E-mail: absalomz2002@gmail.comSearch for more papers by this author First published: 18 March 2015 https://doi.org/10.1002/jmr.2470Citations: 4Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Computational identification of potential epitopes with an immunogenic capacity challenges immunological research. Several methods show considerable success, and together with experimental studies, the efficiency of the algorithms to identify potential peptides with biological activity has improved. Herein, an epitope was designed by combining bioinformatics, docking, and molecular dynamics simulations. The hemagglutinin protein of the H1N1 influenza pandemic strain served as a template, owing to the interest of obtaining a scheme of immunization. Afterward, we performed enzyme-linked immunosorbent assay (ELISA) using the epitope to analyze if any antibodies in human sera before and after the influenza outbreak in 2009 recognize this peptide. Also, a plaque reduction neutralization test induced by virus-neutralizing antibodies and the IgG determination showed the biological activity of this computationally designed peptide. The results of the ELISAs demonstrated that the serum of both prepandemic and pandemic recognized the epitope. Moreover, the plaque reduction neutralization test evidenced the capacity of the designed peptide to neutralize influenza virus in Madin-Darby canine cells. Copyright © 2015 John Wiley & Sons, Ltd. Citing Literature Supporting Information Filename Description jmr2470-sup-0001-figures1.tifTIFF image, 327.2 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume28, Issue9September 2015Pages 553-564 RelatedInformation
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