Resistance of Active Yeast Ribosomes to Dissociation by KCl
1970; Elsevier BV; Volume: 245; Issue: 6 Linguagem: Inglês
10.1016/s0021-9258(18)63264-8
ISSN1083-351X
AutoresTerence E. Martin, Leland H. Hartwell,
Tópico(s)RNA Research and Splicing
ResumoAbstract Monoribosomes deficient in messenger RNA which accumulate in a temperature-sensitive yeast mutant at the restrictive temperature were compared for their dissociability in 0.8 m KCl with polyribosomes and with monoribosomes derived from polyribosomes by ribonuclease treatment. It was found that ribosomes from yeast are dissociated into subunits in 0.8 m KCl only if they lack attached messenger RNA. Ribosomes lacking messenger RNA can be converted to a KCl-stable form by incubation in vitro with transfer RNA, polyuridylic acid, and supernatant.
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