Citotoxidade de diferentes concentrações de hipoclorito de sódio sobre osteoblastos humanos
2009; CENTRO DE PESQUISAS ODONTOLOGICAS SAO LEOPOLDO MANDIC; Volume: 57; Issue: 3 Linguagem: Inglês
ISSN
1981-8637
AutoresTatiana Kelly da Silva Fidalgo, Roberta Barcelos, Debora B. Petropolis, Bruno Rocha Azevedo, Laura Guimarães Primo, Fernando Costa e Silva Filho,
Tópico(s)Dental Trauma and Treatments
ResumoObjective : To evaluate the cytotoxic effect of different amounts of sodium hypochlorite (NaClO), on a culture of human osteoblastos (HOB) cells. Method : Cultures of human osteoblasts (HOB) in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% of bovine fetal serum were incubated in sodium hypochlorite (concentrations of 0.5; 1.0; 2.5 and 5.25%) for 30 seconds. The control group was represented by cells incubated in phosphate buffered saline (PBS). Cell viability was assessed by means of 0.4% trypan blue dye exclusion test, in triplicate. During the incubation period, images were recorded through an inverted optic microscope to evaluate the cellular morphology. Results : In the control group 98.7% of viable cells were verified, without morphology alterations, while no viable cells were observed in the experimental groups. The kinetics of cytotoxity was concentration-dependent. Conclusion : It was concluded that there was a cytotoxic effect on cultures of human osteoblasts incubated for 30 seconds in sodium hypochlorite in all concentrations (0.5; 1.0; 2.5 and 5.25%). Indexing terms : sodium hypochlorite; root canal irrigants; osteoblas; toxicity.
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