The tricarboxylate carrier from rat liver mitochondria
1990; Wiley; Volume: 194; Issue: 2 Linguagem: Inglês
10.1111/j.1432-1033.1990.tb15668.x
ISSN1432-1033
AutoresD. Moira Glerum, Dirk Claeys, Wim Mertens, Angelo Azzi,
Tópico(s)Adipose Tissue and Metabolism
ResumoThe tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A Km of 40 microM and a Vmax of 1.56 mumol x min-1 x mg-1 have been determined for the carrier. Cholesterol levels of between 5-10% of total lipid content are shown to cause a decrease in carrier activity.
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