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Probing poly( N -isopropylacrylamide- co -butylacrylate)/cell interactions by atomic force microscopy

2014; Wiley; Volume: 103; Issue: 1 Linguagem: Inglês

10.1002/jbm.a.35163

1552-4965

Becerra Natalia, Henry Andrade-Caicedo, López Betty, Luz Marina Restrepo Múnera, Roberto Raiteri,

Cellular Mechanics and Interactions

Journal of Biomedical Materials Research Part AVolume 103, Issue 1 p. 145-153 Original Article Probing poly(N-isopropylacrylamide-co-butylacrylate)/cell interactions by atomic force microscopy Becerra Natalia, Becerra Natalia Department of Informatics Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, Via Opera Pia, 13, Genova, Italy Grupo Ciencia de Materiales. Instituto de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia Grupo Ingeniería de Tejidos y Terapia celular Facultad de Medicina Laboratorio Terapia celular y Biobanco, IPS Universitaria, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, ColombiaSearch for more papers by this authorAndrade Henry, Andrade Henry Department of Informatics Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, Via Opera Pia, 13, Genova, Italy Centro de Bioingeniería; Universidad Pontificia Bolivariana; Circular 1 No. 73–76, Bloque 22C, Medellín, ColombiaSearch for more papers by this authorLópez Betty, López Betty Grupo Ciencia de Materiales. Instituto de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, ColombiaSearch for more papers by this authorRestrepo Luz Marina, Restrepo Luz Marina Grupo Ingeniería de Tejidos y Terapia celular Facultad de Medicina Laboratorio Terapia celular y Biobanco, IPS Universitaria, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, ColombiaSearch for more papers by this authorRaiteri Roberto, Corresponding Author Raiteri Roberto Department of Informatics Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, Via Opera Pia, 13, Genova, ItalyCorrespondence to: R. Roberto; e-mail: [email protected]Search for more papers by this author Becerra Natalia, Becerra Natalia Department of Informatics Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, Via Opera Pia, 13, Genova, Italy Grupo Ciencia de Materiales. Instituto de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia Grupo Ingeniería de Tejidos y Terapia celular Facultad de Medicina Laboratorio Terapia celular y Biobanco, IPS Universitaria, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, ColombiaSearch for more papers by this authorAndrade Henry, Andrade Henry Department of Informatics Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, Via Opera Pia, 13, Genova, Italy Centro de Bioingeniería; Universidad Pontificia Bolivariana; Circular 1 No. 73–76, Bloque 22C, Medellín, ColombiaSearch for more papers by this authorLópez Betty, López Betty Grupo Ciencia de Materiales. Instituto de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, ColombiaSearch for more papers by this authorRestrepo Luz Marina, Restrepo Luz Marina Grupo Ingeniería de Tejidos y Terapia celular Facultad de Medicina Laboratorio Terapia celular y Biobanco, IPS Universitaria, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, ColombiaSearch for more papers by this authorRaiteri Roberto, Corresponding Author Raiteri Roberto Department of Informatics Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, Via Opera Pia, 13, Genova, ItalyCorrespondence to: R. Roberto; e-mail: [email protected]Search for more papers by this author First published: 12 March 2014 https://doi.org/10.1002/jbm.a.35163Citations: 7Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Poly(N-isopropylacrylamide) based hydrogels have been proposed as cell culture supports in cell sheet engineering. Toward this goal, we characterized the poly(N-isopropylacrylamide-co-butylacrylate) copolymer thermo-sensitivity and the cell/copolymer interactions above and below the copolymer lower critical solution temperature. We did that by direct force measurements at different temperatures using an atomic force microscope with either a polystyrene or a glass microbead as probes. We used a copolymer-coated microbead to measure adhesion after a short contact time with a single fibroblast in culture. Statistical analysis of the maximum adhesion force and the mechanical work necessary to separate the probe from the cell surface confirmed the hydrophilic/hydrophobic behavior of poly(N-isopropylacrylamide-co-butylacrylate) as a function of temperature in the range 20–37°C and, consequently, a reversible increase/decrease in cell adhesion with the copolymer. As control experiments we measured interactions between uncoated microbeads with the copolymer hydrogel or cells as well as interaction of the Poly(N-isopropylacrylamide) homopolymer with cells. These results show the potential of an assay based on atomic force microscopy for an in situ and quantitative assessment of cell/substrate interactions and support the use of poly(N-isopropylacrylamide-co-butylacrylate) copolymer as an efficient culture substrate in cell sheet engineering. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 145–153, 2015. REFERENCES 1 Cho EC, Lee J, Cho K. Role of bound water and hydrophobic interaction in phase transition of poly(N-isopropylacrylamide) aqueous solution. Macromolecules 2003; 36: 9929–9934. 10.1021/ma034851d CASWeb of Science®Google Scholar 2 Serra L, Doménech J, Peppas NA. Engineering design and molecular dynamics of mucoadhesive drug delivery systems as targeting agents. 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