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A common trans-acting factor, Ad4-binding protein, to the promoters of steroidogenic P-450s.

1992; Elsevier BV; Volume: 267; Issue: 25 Linguagem: Inglês

10.1016/s0021-9258(19)37129-7

1083-351X

Ken-ichirou Morohashi, Shinichi Honda, Yuji Inomata, Hiroshi Handa, Tsuneo Omura,

Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities

Genesmoter regions of all steroidogenic P-450 genes.Furthermore, Ad4BP is expressed only in the cells of steroidogenic organs.These results suggested that Ad4BP is a common factor for the regulation of steroidogenic P-450 genes. MATERIALS AND METHODSOligonucleotides-Oligonucleotides for probes or competitors used in gel shift analyses were synthesized with an Applied Biosystems 380B synthesizer (Applied Biosystems, Foster City, CA).All the oligonucleotides synthesized are listed in Tables I and11.Adl, Ad2, Ad3, Ad4, Ad5, and Ad6 sequences are from bovine CYPllBl gene promoter (19-21).B11-a, B11-b, B11-c, B11-d, and B11-e are also from bovine CYPllBl gene promoter.*H17-a, H17-b, H17-c, and H17-d are from human CYP17 gene promoter (12, 23).H21-a, H21b, and H21-c are from human CYP21 gene promoter (24).HSC-a, HSC-b, HSC-c, HSC-d, HSC-e, HSC-f, HSC-g, and HSC-h are from human CYPIlA gene promoter (7, 25).MSC-a and MSC-b are from mouse CypllA gene promoter (6).HARM-a is from human CYPl9 gene promoter (13).MS4, MS4-a, MS4-b, MS4-c, MS4-d, MS4-e, MS4-f, and MS4-g are model sequences for characterizing the sequence specificity of the binding of purified Ad4BP.The nucleotides