Artigo Acesso aberto Revisado por pares

Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B

1992; American Society for Microbiology; Volume: 174; Issue: 13 Linguagem: Inglês

10.1128/jb.174.13.4509-4512.1992

ISSN

1098-5530

Autores

Christopher R. Meyer, Prachetash Ghosh, Elaine Remy, J. Preiss,

Tópico(s)

Protein Structure and Dynamics

Resumo

A mutant glgC gene contained in a 10.9-kb PstI fragment was cloned from the Escherichia coli B strain SG5 via colony hybridization by using a wild-type glgC probe. The altered allosteric properties of the expressed ADPglucose synthetase were found to result from the conversion of proline to serine at amino acid residue 295.

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