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A simple, efficient method for the separation of humic substances and DNA from environmental samples

1997; American Society for Microbiology; Volume: 63; Issue: 12 Linguagem: Inglês

10.1128/aem.63.12.4993-4995.1997

1098-5336

Colin R. Jackson, Jennifer Paige Harper, D. A. Willoughby, Eric Roden, Perry F. Churchill,

Radioactive contamination and transfer

Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified by the other materials.