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[75] Acid-stable proteinse inhibitors from human seminal plasma

1976; Academic Press; Linguagem: Inglês

10.1016/s0076-6879(76)45078-4

1557-7988

Hans Schießler, Edwin Fink, Hans Fritz,

Phytase and its Applications

Antitryptic activity in human seminal plasma was first detected by Rasmussen and Albrechtson. Haendle et al. described the occurrence of acid-stable trypsin inhibitors in testes, epididymis, and seminal vesicles as well as in the seminal plasma of many mammals, including man. Fink et al. and Suominen and Niemi showed that the antitryptic activity in human seminal plasma is due to two different trypsin inhibitots, the human seminal plasma inhibitors (HUSI) I and II. HUSI-I, trypsin-chymotrypsin inhibitor, and HUSI-II, a trypsin-acrosin inhibitot, were further characterized in laboratory. α-Antitrypsin, an acid-unstable glycoprotein, is also present in human seminal plasma. Trypsin inhibitors isolated from human spermatozoa show very similar characteristics to those of HUSI-I and HUSI-II. This chapter discusses the assay methods like: Trypsin Inhibition, Chymotrypsin Inhibition and Acrosin Inhibition. It also discusses the purification procedure and properties.