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Determination of nicorandil in plasma using high-performance liquid chromatography with photoconductivity and ultraviolet detection

1990; Elsevier BV; Volume: 525; Linguagem: Inglês

10.1016/s0378-4347(00)83388-5

1872-812X

Francis J. Schwende, Richard C. Lewis,

Antibiotics Pharmacokinetics and Efficacy

A sensitive and precise method for the determination of nicorandil, a new anti-anginal medication, is reported. The method involves solid-phase extraction of the drug and internal standard using Bond-Elut® C18 extraction columns, reversed-phase high-performance liquid chromatography on a Zorbax®-Phenyl column and detection with photoconductivity and ultraviolet detection in series. Photoconductivity, performed with the Tracor 965 photoconductivity detector, provided a limit of detection of 2 ng/ml in plasma (between-day coefficient of variation of 15%) but the linear range of response was limited to only about 100 ng/ml. Ultraviolet detection in series with the photoconductivity detector extended the linear range of the analytical system to 1000 ng/ml (coefficient of variation 4.4%). The utility of the method is demonstrated in a dog pharmacokinetic study in which a 5-mg intravenous dose was compared to a 10-mg oral solution dose in six beagle dogs. The oral solution was absorbed rapidly, achieving an average maximum concentration of 857 ng/ml in 11.2 min. The absolute bioavailability of nicorandil in dogs in this study was determined to be 84.2%