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Time-resolved fluorometry for the identification of viral DNA in clinical specimens

1988; American Society for Microbiology; Volume: 26; Issue: 11 Linguagem: Inglês

10.1128/jcm.26.11.2434-2436.1988

1098-660X

Patrik Dahlén, Pertti Hurskainen, Timo Lövgren, Timo Hyypiä,

Advanced biosensing and bioanalysis techniques

Time-resolved fluorometry was used for the detection of DNA probes labeled directly with europium (Eu3+) chelate. The sensitivity of this nonisotopic hybridization assay was 100 pg of homologous DNA. Equivalent results with reference tests were obtained in the detection of adenoviruses in clinical specimens.